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概述

  • 產品名稱

  • 描述

    兔多克隆抗體to PDX1
  • 宿主

    Rabbit
  • 經測試應用

    適用于: IHC-Fr, WB, ICC/IF, IHC-Pmore details
  • 種屬反應性

    與反應: Mouse, Rat, Cow, Dog, Human
  • 免疫原

    Recombinant fusion protein containing N-Terminal region of mouse Pdx1

應用

Our Abpromise guarantee covers the use of ab47267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Ab評論 說明
IHC-Fr 1/5000.
WB 1/3000 - 1/5000. Predicted molecular weight: 31 kDa.
ICC/IF 1/2000.

See ICC-specific protocol in protocol tab.

IHC-P Use at an assay dependent concentration.

See IHC-specific protocol in protocol tab.

靶標

  • 功能

    Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell.
  • 組織特異性

    Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells).
  • 疾病相關

    Defects in PDX1 are a cause of pancreatic agenesis (PAC) [MIM:260370]. This autosomal recessive disorder is characterized by absence or hypoplasia of pancreas, leading to early-onset insulin-dependent diabetes mellitus. This was found in a frameshift mutation that produces a truncated protein and results in a second initiation that produces a second protein that act as a dominant negative mutant.
    Defects in PDX1 are a cause of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
    Defects in PDX1 are the cause of maturity-onset diabetes of the young type 4 (MODY4) [MIM:606392]; also symbolized MODY-4. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
  • 序列相似性

    Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 結構域

    The Antp-type hexapeptide mediates heterodimerization with PBX on a regulatory element of the somatostatin promoter.
    The homeodomain, which contains the nuclear localization signal, not only mediates DNA-binding, but also acts as a protein-protein interaction domain for TCF3(E47), NEUROD1 and HMG-I(Y).
  • 翻譯后修飾

    Phosphorylated by the SAPK2 pathway at high intracellular glucose concentration.
  • 細胞定位

    Nucleus.
  • Information by UniProt
  • 數據庫鏈接

  • 別名

    • Glucose sensitive factor antibody
    • Glucose-sensitive factor antibody
    • GSF antibody
    • IDX 1 antibody
    • IDX-1 antibody
    • IDX1 antibody
    • Insulin promoter factor 1 antibody
    • Insulin promoter factor 1 homeodomain transcription factor antibody
    • Insulin upstream factor 1 antibody
    • IPF 1 antibody
    • IPF-1 antibody
    • IPF1 antibody
    • Islet/duodenum homeobox 1 antibody
    • Islet/duodenum homeobox-1 antibody
    • IUF 1 antibody
    • IUF-1 antibody
    • IUF1 antibody
    • MODY4 antibody
    • Pancreas/duodenum homeobox 1 antibody
    • Pancreas/duodenum homeobox protein 1 antibody
    • pancreatic and duodenal homeobox P antibody
    • PDX 1 antibody
    • PDX-1 antibody
    • PDX1 antibody
    • PDX1_HUMAN antibody
    • Somatostatin transactivating factor 1 antibody
    • Somatostatin-transactivating factor 1 antibody
    • STF 1 antibody
    • STF-1 antibody
    • STF1 antibody
    see all

圖片

  • Immunohistochemical analysis of murine pancreatic islets, staining PDX1 (red) with ab47267.

    Tissue was fixed in formalin, permeablized with 0.25% Triton X-100, and blocked in blocking solution for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody overnight at 4°C and a fluorescent-conjugated anti-rabbit IgG was used as the secondary antibody.
  • ab47267 staining PDX1 in rat pancreatic tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C. The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. 

    See Abreview

  • ab47267 staining PDX1 in embryonic (day 13.5) mouse pancreas tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with formaldehyde and blocking with 10% serum for 30 minutes at 220C was performed. The sample was incubated with primary antibody (1/5000) in 10% serum for 16 hours in 220C. A biotin-conjugated donkey polyclonal to rabbit IgG was used at dilution at 1/250 as secondary antibody. A biotin/avidin/streptavidin linked to HRP system has been used.

    See Abreview

  • Immunostaining of islet-epithelial clusters from human foetal pancreata with ab47267 (green). Nuclei are visualised with DAPI (blue).
  • ab47267 staining PDX1 in human appendix tissue sections by immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in citrate buffer pH6.0 and blocking for 15 minutes at 20°C (5 minutes for peroxide block and 10 minutes for protein block). The primary antibody was diluted 1/500 and incubated with sample for 45 minutes at 20°C. A HRP-conjugated goat polyclonal to rabbit IgG was used undiluted as the secondary. 

文獻

This product has been referenced in:

  • Yuan F  et al. Effects of Huanglian-Renshen-Decoction, a Fixed Mixture of Traditional Chinese Medicine, on the Improvement of Glucose Metabolism by Maintenance of Pancreatic ? Cell Identity in db/db Mice. Evid Based Complement Alternat Med 2019:1232913 (2019). Read more (PubMed: 31015847) ?
  • Barati G  et al. Differentiation of microfluidic-encapsulated trabecular meshwork mesenchymal stem cells into insulin producing cells and their impact on diabetic rats. J Cell Physiol 234:6801-6809 (2019). Read more (PubMed: 30317587) ?
See all 53 Publications for this product

客戶評價及客戶問答

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1-10 of 14 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na Citrate pH 6.0
Permeabilization
No
Specification
Pancreas
Fixative
10% NBF

Dr. David Burk

Verified customer

提交于 Nov 08 2018

Application
Western blot
Sample
Human Cell lysate - whole cell (AML-193)
Gel Running Conditions
Reduced Denaturing (4-20% Tris Glycin gel, semi dry transfer)
Loading amount
50 µg
Specification
AML-193
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Sep 03 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (pancreas)
Permeabilization
No
Specification
pancreas
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jul 30 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH=9.0
Permeabilization
Yes - PBS with 3% Triton-100
Specification
Pancreas
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Mar 22 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® iPSC Diff Day 5)
Permeabilization
No
Specification
iCell® iPSC Diff Day 5
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

提交于 Sep 17 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Human Cell (H9 cell line upon differentiation)
Specification
H9 cell line upon differentiation
Permeabilization
Yes - 0.01% Triton X100
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Oct 22 2014

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human embryonic stem cell derived-pancreatic endoc)
Specification
human embryonic stem cell derived-pancreatic endoc
Fixative
Formaldehyde
Permeabilization
Yes - 0.1 % Triton x-100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Youngjin Kim

Verified customer

提交于 Oct 25 2012

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Pancreas)
Specification
Pancreas
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Oct 06 2010

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Pancreas (embryonic day 13.5))
Specification
Pancreas (embryonic day 13.5)
Fixative
Formaldehyde
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Sep 08 2009

Application
Western blot
Sample
Mouse Tissue lysate - whole (Ms Pancreas, Ms Skeletal Muscle, Ms Brain)
Loading amount
30 µg
Specification
Ms Pancreas, Ms Skeletal Muscle, Ms Brain
Gel Running Conditions
Reduced Denaturing (12.5% SDS PAGE)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

提交于 Nov 17 2008

1-10 of 14 Abreviews

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