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重組
RabMAb

重組Anti-PDGFR beta抗體[Y92] - C-terminal (ab32570)

概述

  • 產品名稱

    Anti-PDGFR beta抗體[Y92] - C-terminal
    參閱全部 PDGFR beta 一抗
  • 描述

    兔單克隆抗體[Y92] to PDGFR beta - C-terminal
  • 宿主

    Rabbit
  • 特異性

    Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) recognizes human platelet-derived growth factor (PDGF) receptor beta. It does not cross-react with other CSF-1/PDGF receptor family members. Expression levels of the target protein vary with sample type and some optimisation may be required.
  • 經測試應用

    適用于: IHC-Fr, Flow Cyt, IHC-FoFr, WB, IHC-P, ICC/IF, IP, IHC-FrFlmore details
  • 種屬反應性

    與反應: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human PDGFR beta aa 1050 to the C-terminus. The exact sequence is proprietary.
    Database link: P09619

  • 陽性對照

    • WB: NIH/3T3 cell lysate. SH-SY5Y cell lysate. Rat brain and heart tissue lysate. Mouse brain tissue lysate. Human fetal brain tissue lysate. ICC/IF: NIH/3T3 cells. IHC-P: Human prostatic carcinoma, lung cancer, breast and spleen tissue. Flow Cyt: NIH/3T3 cells. IP: NIH/3T3 cell lysate.
  • 常規說明

      

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

應用

Our Abpromise guarantee covers the use of ab32570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

應用 Ab評論 說明
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/20.
IHC-FoFr Use at an assay dependent concentration.
WB 1/5000 - 1/20000. Predicted molecular weight: 124 kDa.Can be blocked with Human PDGFR beta peptide (ab263451).

For samples expressing low levels of PDGFR beta, the amount of lysate loaded may need to be increased to allow detection. 

IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Optimisation of the IHC protocol may be required depending on the sample used.

ICC/IF 1/100.
IP 1/20.
IHC-FrFl Use at an assay dependent concentration. PubMed: 25077029

靶標

  • 功能

    Receptor that binds specifically to PDGFB and PDGFD and has a tyrosine-protein kinase activity. Phosphorylates Tyr residues at the C-terminus of PTPN11 creating a binding site for the SH2 domain of GRB2.
  • 疾病相關

    Note=A chromosomal aberration involving PDGFRB is found in a form of chronic myelomonocytic leukemia (CMML). Translocation t(5;12)(q33;p13) with EVT6/TEL. It is characterized by abnormal clonal myeloid proliferation and by progression to acute myelogenous leukemia (AML).
    Note=A chromosomal aberration involving PDGFRB may be a cause of acute myelogenous leukemia. Translocation t(5;14)(q33;q32) with TRIP11. The fusion protein may be involved in clonal evolution of leukemia and eosinophilia.
    Note=A chromosomal aberration involving PDGFRB may be a cause of juvenile myelomonocytic leukemia. Translocation t(5;17)(q33;p11.2) with SPECC1.
    Defects in PDGFRB are a cause of myeloproliferative disorder chronic with eosinophilia (MPE) [MIM:131440]. A hematologic disorder characterized by malignant eosinophils proliferation. Note=A chromosomal aberration involving PDGFRB is found in many instances of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;12) with ETV6 on chromosome 12 creating an PDGFRB-ETV6 fusion protein.
    Note=A chromosomal aberration involving PDGFRB may be the cause of a myeloproliferative disorder (MBD) associated with eosinophilia. Translocation t(1;5)(q23;q33) that forms a PDE4DIP-PDGFRB fusion protein.
  • 序列相似性

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 翻譯后修飾

    Autophosphorylated. Dephosphorylated by PTPRJ at Tyr-751, Tyr-857, Tyr-1009 and Tyr-1021.
  • 細胞定位

    Membrane.
  • Information by UniProt
  • 數據庫鏈接

  • 別名

    • Beta platelet derived growth factor receptor antibody
    • Beta-type platelet-derived growth factor receptor antibody
    • CD 140B antibody
    • CD140 antigen-like family member B antibody
    • CD140b antibody
    • CD140b antigen antibody
    • IBGC4 antibody
    • IMF1 antibody
    • JTK12 antibody
    • OTTHUMP00000160528 antibody
    • PDGF R beta antibody
    • PDGF Receptor beta antibody
    • PDGF-R-beta antibody
    • PDGFR 1 antibody
    • PDGFR antibody
    • PDGFR beta antibody
    • PDGFR1 antibody
    • PDGFRB antibody
    • PGFRB_HUMAN antibody
    • Platelet derived growth factor receptor 1 antibody
    • Platelet derived growth factor receptor beta antibody
    • Platelet derived growth factor receptor beta polypeptide antibody
    see all

圖片

  • ab32570 staining PDGFR beta in human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Shows positive staining on stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). An HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.

  • All lanes : Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/10000 dilution (purified)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat heart tissue lysate
    Lane 3 : Mouse brain tissue lysate

    Lysates/proteins at 20 μg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa
    why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunofluorescence analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells stimulated with PDGF, staining PDGFR beta with unpurified ab32570.

  • Flow cytometry analysis of NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling PDGFR beta (red) with ab32570 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

  • Immunohistochemical staining of paraffin embedded human spleen with purified ab32570 at a working dilution of 1/50. The secondary antibody used is ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L), at a dilution of 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • Immunofluorescence staining of NIH/3T3 (Mouse embryo fibroblast cell line) cells with purified ab32570 at a working dilution of 1 in 100, counter-stained with DAPI. Tubulin was stained with mouse anti-tubulin at a dilution of 1/1000 (ab7291) and Alexa Fluor® 594 goat anti-mouse at a dilution of 1/500 (ab150120) . The secondary antibody was ab150077 Alexa Fluor® 488 goat anti rabbit, used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100.

    The negative controls are shown in the bottom middle and right hand panels - for the first negative control, purified ab32570 was used at a dilution of 1/200 followed by an Alexa Fluor® 555 goat anti-mouse antibody at a dilution of 1/500 and for the second negative control mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab15007) were used.

  • ab32570 (purified) at 1/20 immunoprecipitating PDGFR beta in NIH/3T3 (Mouse embryo fibroblast cell line) (Lane 1 and 2). Lane 3 - PBS.

    For western blotting a HRP-conjugated anti-rabbit IgG specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

  • Human aortic valve: immunofluorescence labelling shows telocytes.

    Frozen sections were sliced into a thickness of 6 μM, and then were post‐fixed with 4% paraformaldehyde dissolved in 0.1 M phosphate buffer (pH = 7.4) for at least 15 min. After washed with phosphate buffer for three times, sections were immersed in 10% goat serum for 1 hr. After that, sections were incubated overnight at 4°C with rat monoclonal anti‐CD34 (ab8158; Abcam, Cambridge, UK) and either rabbit monoclonal to Vimentin (ab92547; Abcam), rabbit monoclonal to PDGF Receptor‐beta (ab32570, 1:100; Abcam) or rabbit polyclonal anti‐C‐Kit (ab5506, 1:100; Abcam), both were with the dilution of 1:100 in phosphate buffer and permeabilized by 0.25% Triton X‐100 at the same time. On the second day, the sections were incubated to goat anti‐rabbit labelled with rhodamine secondary antibodies and goat anti‐rat labelled with FITC diluted 1:200 in phosphate buffer for 1 hr. The sections were stained with 4′,6‐diamidino‐2‐phenylindole (DAPI). 

  • Newborn rat pups received a single injection of GA (1 μmol/g) or vehicle into the cisterna magna. they were analysated analyzed at 14 and 30 days post injection (DPI) Characterization of EB positive cells was made by double labeling with ab32570 an antibody to β receptor of the platelet derived growth factor (PDGFRβ) at a dilution of 1/100.

    Immunohistochemistry assays were performed on free-floating sections. Sections were washed with PBS; permeabilized 20 min with 0.05% Triton X-100 and incubated 30 min in blocking buffer (PBS containing 0.05% Triton X-100 and 5% bovine serum albumin (BSA)).

    Representative images of the immunoreactivity for the pericyte marker PDGFRβ evidencing strong signal around some blood vessels (white arrows) and some middle-sized positive cells (arrowheads). At 30 DPI, there was a reduction in the PDGFRβ signal around blood vessels of GA-injected ratwhen compared to controls.

  • Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/10000 dilution (purified) + SH-SY5Y (Human neuroblastoma cell line from bone marrow) cell lysate at 10 μg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/5000 dilution (purified) + Human fetal brain tissue lysate at 10 μg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Anti-PDGFR beta antibody [Y92] - C-terminal (ab32570) at 1/50000 dilution (purified) + NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate at 10 μg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 124 kDa
    Observed band size: 175 kDa why is the actual band size different from the predicted?



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • ab32570 staining PDGFR beta in human breast tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Shows positive staining on stromal cells. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/500). An HRP-conjugated Goat anti-rabbit IgG (ready to use) was used as the secondary antibody. Counter stained with Hematoxylin.

文獻

This product has been referenced in:

  • Perin S  et al. Isolation and characterisation of mouse intestinal mesoangioblasts. Pediatr Surg Int 35:29-34 (2019). IHC (PFA fixed), ICC/IF ; Mouse . Read more (PubMed: 30406837) ?
  • Greenhalgh SN  et al. Loss of Integrin av?8 in Murine Hepatocytes Accelerates Liver Regeneration. Am J Pathol 189:258-271 (2019). IHC-P ; Mouse . Read more (PubMed: 30448409) ?
See all 148 Publications for this product

客戶評價及客戶問答

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1-10 of 23 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Liver
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 4°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Oct 24 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Oct 24 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Oct 24 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
Permeabilization
Yes - 0.05% Tween 20
Specification
Kidney
Blocking step
sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Oct 24 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryonic tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer
Permeabilization
No
Specification
embryonic tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 7% · Temperature: 27°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

提交于 Jul 10 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, mesangial)
Permeabilization
No
Specification
kidney, mesangial
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Paraformaldehyde

Miss. Kara Shumansky

Verified customer

提交于 Mar 04 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (SH-SY5Y cell line)
Permeabilization
No
Specification
SH-SY5Y cell line
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 3%
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Feb 26 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Methanol

Abcam user community

Verified customer

提交于 Aug 09 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 5%
Fixative
Methanol

Dr. gozde uzunalli

Verified customer

提交于 Aug 09 2018

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (Spleen, liver, intestine and kidney)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH9
Permeabilization
No
Specification
Spleen, liver, intestine and kidney
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
Fixative
Formaldehyde

Victor Bernal

Verified customer

提交于 Mar 19 2018

1-10 of 23 Abreviews

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