產品名稱人 Cardiotoxicity FirePlex®-HT組合1
樣品類型Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
Human Cardiotoxicity FirePlex®-HT Panel 1 (ab252375) uses FirePlex® particle technology to quantify ICAM1, Cardiac Troponin I, FABP3, NT-proBNP, and BNP from 6.25 µl of sample input in 384-well plate format.
Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.
The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.
Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere
This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.
The following table provides the sensitivity, dynamic range, and CVs for Human Cardiotoxicity FirePlex®-HT Panel 1 *.
BNP 8.71 13.72 - 3,333 13.3% Cardiac Troponin I∇ 92.60 123.5 - 30,000 9.2% FABP3∇ 6.42 13.72 - 1,111 8.6% ICAM1 26.79 41.15 - 30,000 10.7% NT-proBNP 15.53 41.15 - 3,333 11.7%
*representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.
∇The bovine and human proteins for these analytes exhibit a >90% sequence homology, thus some bovine serum cross-reactivity may be observed in cell culture supernatant (CCS) samples. To address this, we strongly recommend that users include a cell culture media-only control in experiments using CCS samples.
存放說明Please refer to protocols.
組件 1 units 3 units 384 Well Imaging Plate 1 x 384 tests 3 x 384 tests 384 Well Imaging Plate Lid 1 unit 3 units 384 Well Imaging Plate Seal 2 units 6 units FirePlex-HT 1X PBS 1 x 8ml 3 x 8ml FirePlex-HT- 5X Capture Particle Mix 1 x 700μl 3 x 700μl FirePlex-HT Human Protein Standard Mix HTCTX 1 x 7 tests 3 x 7 tests FirePlex-HT Reagent Diluent R1 1 x 8ml 3 x 8ml FirePlex-HT Sample Diluent S1 1 x 25ml 3 x 25ml FirePlex-HT - Imaging Dye Concentrate 1 x 875μl 3 x 875μl FirePlex-HT 35X Detector Antibody Mix 1 x 110μl 3 x 110μl
ab252375 has not yet been referenced specifically in any publications.