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FirePlex

人 Cardiotoxicity FirePlex®-HT組合1 (ab252375)

概述

  • 產品名稱

    人 Cardiotoxicity FirePlex®-HT組合1
  • 檢測方法

    Fluorescent
  • 樣品類型

    Cell culture supernatant, Serum, Plasma, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • 檢測類型

    Multiplex
  • 種屬反應性

    與反應: Human
  • 產品概述

    Human Cardiotoxicity FirePlex®-HT Panel 1 (ab252375) uses FirePlex® particle technology to quantify ICAM1, Cardiac Troponin I, FABP3, NT-proBNP, and BNP from 6.25 µl of sample input in 384-well plate format.


    Assays are validated in serum, plasma, and cell culture supernatant, and can be run with 175 samples in duplicate per 384-well plate.


    The two-step workflow and no-wash assay format limit hands-on time and are amenable to automation, thus making FirePlex-HT ideally suited for high-throughput screening studies. Assay readout is conducted on high-content imagers with data analysis using the FirePlex® Analysis Workbench software.


    Users should note that assay performance data supplied in the accompanying Product Datasheet is a representative dataset, and is not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.


    FirePlex® is a registered trade mark in certain territories (including Europe and the United States) and is an unregistered trade mark elsewhere

  • 說明

    This product is currently only supported for customers in North America and Europe, if you are outside these areas please contact us.

    The following table provides the sensitivity, dynamic range, and CVs for Human Cardiotoxicity FirePlex®-HT Panel 1 *.

    Target 

    Sensitivity/LOD

    (pg/ml) 

    Dynamic Range

    (pg/ml) 

     Inter-well CV

    (Std curve)

    BNP  8.71 13.72 - 3,333  13.3%
    Cardiac Troponin I  92.60 123.5 - 30,000 9.2% 
    FABP3  6.42 13.72 - 1,111 8.6%
    ICAM1  26.79 41.15 - 30,000 10.7%
    NT-proBNP  15.53 41.15 - 3,333 11.7%

    *representative dataset, not lot-specific. For lot-specific assay performance data, please refer to the product's certificate of analysis, which can be found here.

    The bovine and human proteins for these analytes exhibit a >90% sequence homology, thus some bovine serum cross-reactivity may be observed in cell culture supernatant (CCS) samples. To address this, we strongly recommend that users include a cell culture media-only control in experiments using CCS samples.

  • 平臺

    Multiplex

性能

  • 存放說明

    Please refer to protocols.
  • 組件 1 units 3 units
    384 Well Imaging Plate 1 x 384 tests 3 x 384 tests
    384 Well Imaging Plate Lid 1 unit 3 units
    384 Well Imaging Plate Seal 2 units 6 units
    FirePlex-HT 1X PBS 1 x 8ml 3 x 8ml
    FirePlex-HT- 5X Capture Particle Mix 1 x 700μl 3 x 700μl
    FirePlex-HT Human Protein Standard Mix HTCTX 1 x 7 tests 3 x 7 tests
    FirePlex-HT Reagent Diluent R1 1 x 8ml 3 x 8ml
    FirePlex-HT Sample Diluent S1 1 x 25ml 3 x 25ml
    FirePlex-HT - Imaging Dye Concentrate 1 x 875μl 3 x 875μl
    FirePlex-HT 35X Detector Antibody Mix  1 x 110μl 3 x 110μl

圖片

實驗方案

文獻

ab252375 has not yet been referenced specifically in any publications.

客戶評價及客戶問答

There are currently no Customer reviews or Questions for ab252375.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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